Electron microscopy and image processing

Institute of Structural and Molecular Biology
Birkbeck College and University College London

Dr Natasha Lukoyanova tel: (office) +44(0) 207 631 6824 n.lukoyanova [at] bbk.ac.uk Dr Shu Chen Office +44(0) 207 631 6831 EM unit +44(0) 207 079 8058 EM preparation lab +44(0) 207 079 8061; T12 x8057; T10 x8058 s.chen [at] bbk.ac.uk

EM lab safety EM Wiki Local EM information Booking system	Academic Head of Laboratory: Carolyn Moores Laboratory Co-ordinator: Natasha Lukoyanova Laboratory Manager: Shu Chen EM groups Helen Saibil Giulia Zanetti Anthony Roberts Finn Werner Elena Orlova Gabriel Waksman Phil Robinson Jerome Gouge		Course Files 2019

EM equipment
For single particle EM and tomography, we have Tecnai 100, 120 kV, 200 kV FEG and 300 kV Krios machines, with Falcon 3 and K3 direct electron detectors and BioQuantum energy filter and phase plate on the  Krios, a DE20 direct detector on the F20, and a Vitrobot and Leica Gp2 for sample preparation. Cell preparation equipment includes a high pressure freezer, cryo microtome and freeze substitution, as well as correlative cryo fluorescence microscopy/ cryo EM.

Photos of a challenging Krios delivery

Image processing software:
Spider Imagic Amira CCP4 CCP-EM EMAN IMOD Dynamo RELION Cryosparc

eBIC: electron Bio-Imaging Centre at Diamond Light Source